Immobilization and orientation control of functional proteins on the surfaces of materials
has been regarded as a key factor for enhancement of performance of protein-based
materials in the fields of bio-production, diagnostics and clinical treatments. Especially,
Sensitive and accurate biomolecular sensing utilizing antibody-immobilized solid support of
the solid-sensing materials are highly expected in clinical diagnosis, pharmaceutical
process monitoring and environmental analysis. Here, we propose and demonstrate
development of materials-binding peptides for site-specific immobilization of recombinant
antibody fragments, such as single-chain Fv antibodies and single-domain antibodies
(VHH).